Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak

نویسندگان

  • Wan Beom Park
  • Nak-Jung Kwon
  • Pyoeng Gyun Choe
  • Su-Jin Choi
  • Hong Sang Oh
  • Sang Min Lee
  • Hyonyong Chong
  • Jong-Il Kim
  • Kyoung-Ho Song
  • Ji Hwan Bang
  • Eu Suk Kim
  • Hong-Bin Kim
  • Sang Won Park
  • Nam Joong Kim
  • Myoung-Don Oh
چکیده

During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

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عنوان ژورنال:

دوره 31  شماره 

صفحات  -

تاریخ انتشار 2016